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Mathematisch-Naturwissenschaftliche Fakultät - Jahrgang 2008



Actin cytoskeleton and endomembrane system dependent cell growth


Boris Voigt




In plants, membrane recycling processes are involved in every instance of cell volume changes which occur during growth, development and adaptation to the environment. The thesis work described here addresses membrane recycling on three different levels. (i) The level of the actin cytoskeleton as the major cytoskeletal structure, which supports vesicle trafficking in plant cells. (ii) The level of endosomal dynamics as the major membrane compartment that is involved in membrane recycling at the plasma membrane and (iii) at the level of macromolecular components, which are involved in vesicle fusion.
1) The actin cytoskeleton: A GFP-based actin cytoskeleton reporter construct has been designed, GFP-FABD2, on the basis of the plant fimbrin 1 sequence, which proved to be very reliable and useful as a faithful reporter of the actin cytoskeleton in transgenic A. thaliana lines. With this new fluorescent reporter, the structure and dynamic of the actin cytoskeleton is studied in greater detail in correlation with the motile behaviour of endosomal compartments. more...

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© Universitäts- und Landesbibliothek Bonn | Veröffentlicht: 2008