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Mathematisch-Naturwissenschaftliche Fakultät - Jahrgang 2007


Titel Therapy Approach of Neurodegenerative Disorders by Bone Marrow Stem Cells
Autor Sara Alejandra Litwak
Publikationsform Dissertation
Abstract Multiple Sclerosis and cerebral ischemia are severe neurodegenerative diseases. Treatment in both cases is inadequate as drug delivery to the central nervous system (CNS) represents a difficulty due to the presence of the blood-brain-barrier (BBB) and its control of molecule trafficking. The migration and integration into CNS parenchyma of adult stem cell coming from bone marrow (BM) has been widely reported suggesting their potential as cell therapy themselves, but also as means for local delivery of therapeutic agents.
In this work, the isolation of a cell population derived from the mouse bone marrow (BM), which is enriched in hematopoietic progenitor/stem cells (termed Lin-HSC) and their application in two neurodegenerative paradigms: the animal model for multiple sclerosis, Experimental Autoimmune Encephalomielitis (EAE), and the animal model for cerebral ischemia were described. Furthermore, the use of these cells as delivery system for a potential therapeutic factor, Neurotrophin-3 was studied. Lin-HSC were isolated and characterized by flow cytometry and quantitative real time PCR. Lin-HSC showed no expression of blood lineage differentiated cells neither of the receptors and integrins, but were positive for c-kit (29%) and CD45 (58%). more...
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© Universitäts- und Landesbibliothek Bonn | Veröffentlicht: 2007